Monday, 8 August 2016

Cannabis Smoke Causes Up-Regulation of Akt and Bax Protein in Subfertile Patients Sperm Cells

Emerging worldwide evidences in support of adverse effects of cannabis smoke indicate its significant role in declining male fertility. The aim of the present study was to compare the percentage of damaged sperm cells and the expression profiles of cell survival protein p-Akt and pro-apoptotic protein Bax in non-smoker, tobacco smoke addicted and cannabis smoke addicted subfertile subjects.

Since last few decades, a sharp decline in male fecundity has been observed all over the world. In addition to congenital abnormalities, environmental and occupational exposures, changed lifestyle factors were also found to impact male reproductive health. Several studies reported direct association of excessive tobacco and alcohol consumption with the declining male fertility . Emerging studies have also correlated addiction to cannabis smoke with poor semen quality of men.Contents of cannabis smoke reduce antioxidant defence mechanism and increase oxidative stress in seminal plasma.
It has been estimated that around 150 million people across the globe were addicted to cannabis in the beginning of this millennia .The hallucinogenic effects caused by this recreational drug, entices people of different age groups and socio-economic classes in different countries all over the world. ‘Cannabis’ is a generic term used for marijuana, hashish and hash oil and it is derived from the Cannabis sativa plant. Δ9-Tetrahydrocannabinol (THC) is the unique compound of cannabis with major psychoactive effects and is said to act upon a specific cannabinoid receptor (CB1) in the brain.

In the 1990s, it was observed that cannabinoid compounds are naturally synthesized in human body from fatty acid derivatives termed as endogenous cannabinoids or endocannabinoids . Endocannabinoids modulate several pathophysiologic processes like neuropathic pain, movement disorders such as Parkinson disease, Huntington disease and many other conditions like atherosclerosis, obesity as well as reproductive health. However, the association between cannabis smoking and cancer is highly disputed as different case-control studies had inferred different result. Both cannabinoid and nicotine  receptors are coupled to the protein kinase B (Akt) signalling pathway. Akt is a serine threonine kinase which induces anti-apoptotic signal and inhibits apoptosis. However, the role of Akt coupled with cannabinoid receptor, varies from one disease to another

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